Random Allocation in Experiment

A true experiment is one where each subject is allotted to truly random conditions. In this sense, we cannot perform a true experiment. Never in the history of science has a true experiment been performed. Only the multiverse itself can be considered a condition of random allotment. If one could stand outside the many branches that remain spawned from every observation-like event, then a true experiment may be witnessed. Switch spin up to spin down and behold.

As researchers, we seek random allocation so that we can make strong inferences about causal relationships. The only reason for random allocation is because the subjects we deal with cannot be paused, copied, and pasted to a different condition. It is due to the makeshift nature of our subject clones that we are compelled to close our eyes and cast them in randomness. Randomness is the craving to recover the simple, the veil to hide the shame that we could not be gods.

Tolerogenic transcriptome landscape in CD8+ T lymphocytes after exposure to erythrocyte-targeted antigens

Mice in research.

Tolerogenic transcriptome landscape in CD8+ T lymphocytes after exposure to erythrocyte-targeted antigens

First, you must be approved by regulatory authority, since you will be working with animals.

The kind they used was Male C57BL/6 mice. The age was 8-12 weeks.

These can be ordered through Envigo. If you are in America, the cost is not specified on the website for mysterious reasons. However, if you are in Italy, for example, it is perfectly clear that you will pay 24.30 euros per mice, with the price increasing as they get older.

CD45.1+ OTI mice were generated by crossing C57BL/6-Tg (TcraTcrb) 1100Mjb (OTI) mice (Jackson Laboratories) with C57BL/6-Ly5.1 (Charles River).

C57BL/6-Tg (TcraTcrb) 1100Mjb (OTI) don’t seem to be available at Jackson Laboratories. But the similar C57BL/6-Tg(TcraTcrb)1100Mjb/J and cost anywhere from $223.09 to $243.09 based on the age (3 weeks-9 weeks).

The Charles River strain asks me to sign up just to see the price, so I will not be pursuing that.